Logo - Rejuvenal
Cartilage from shark or ray
Cartilage from fish and our health
Our shark cartilage has many more benefits for our body than just being the carrier material for the Peptide! Known are the benefits for joints and cartilage. But also for skin quality, shark cartilage plays an important role, because of the content of Hyaluronic acid, Chondroitin Sulfate and Glycosaminoglycans. And, as the next very recent article from PubMed will show, it has also many benefits for the quality of our blood! Crucial however is the technology used to derive these delicate but potent ingredients from the source material.
Direct-acting fibrinolytic enzymes in shark cartilage extract: potential therapeutic role in vascular disorders.
Ratel D, Glazier G, Provencal M, Boivin D, Beaulieu E, Gingras D, Beliveau R.Laboratoire de Medecine Moleculaire Ste-Justine-UQAM, Centre de Cancerologie Charles-Bruneau, Hopital Ste-Justine et Universite du Quebec a Montreal, 3175 Chemin Cote-Ste-Catherine, Montreal, QC, Canada H3T 1C5. Thromb Res. 2005;115(1-2):143-52.
Fibrinogen and fibrin are molecules with overlapping roles in blood clotting, fibrinolysis, wound healing, inflammation, matrix and cellular interactions and neoplasia. There is currently much interest in the possible use of fibrinolytic agents in human therapeutics. In this study, we report the presence of fibrinolytic activities in shark cartilage extract (SCE). In vitro, SCE at 100 microg/ml completely degraded fibrin gel in an aprotinin-insensitive manner, suggesting a non-plasmin molecular nature. SCE was able to cleave all chains of fibrinogen and fibrin and the cleavage was completely inhibited by 1,10-phenanthroline, suggesting an essential role for metalloprotease(s) in this process.
Using fibrinogen zymography, we show that SCE contains two plasmin-independent fibrinolytic activities and that these activities are correlated with the presence of 58 and 62 kDa proteases in the extract. SCE-fibrinolytic activities are inhibited by dithiothreitol, suggesting that disulfide bonds are necessary for the protease structure. Finally, using thromboelastography, SCE markedly induced retraction of human platelet-rich plasma (PRP) clot, this process being completely abolished by 1,10-phenanthroline.
These data suggest the presence of novel non-plasmin fibrinolytic activities within Shark Cartilage Extract. This extract may thus represent a potential source of new therapeutic molecules to prevent and treat vaso-occlusive and thromboembolic disorders.
Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(4):325-8.
Effect of Ray cartilage glycosaminoglycans (RCG) on the expression of MMP-9 in Lewis lung carcinoma
Guo B, Han GY, Li Z.
Basic Medical College, China Medical University, Shenyang 110001, China.
OBJECTIVE: To explore the effect of Ray cartilage glycosaminoglycans (RCG) on the expression of MMP-9 in Lewis lung carcinoma of mice.
METHOD: The model of mice with Lewis lung carcinoma was induced. The experimental mice were randomly divided into normal saline group, RCG groups at varied concentrations and CTX group. Tumor growth state was observed, and tumor inhibitory rate of primary tumor and number of lung metastasis focus were measured. The expression of MMP-9 mRNA and protein in Lewis lung carcinoma was determined with RT-PCR and Western blot.
RESULT: As compared with normal saline group, tumor growth curves in RCG groups were smooth, there were significant differences of inhibitory rates of primary tumor and number of lung metastasis focus between RCG groups and normal saline group, and MMP-9 mRNA and protein expression levels in RCG groups were reduced significantly.
CONCLUSION: RCG can inhibit effectively the growth and metastasis of implanted Lewis lung carcinoma in C57BL/6 mice, which is probably attributed to reducing the expression of MMP-9 mRNA and protein.
Studie1