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Skin improvement and wrinkle reduction
The skin improving, lipid-lowering and antioxidative effects of marine collagen peptides
Wang JB, Xie Y, Pei XR, Yang RY, Zhang ZF, Li Y. Department of Nutrition and Food Hygiene, School of Public Health, Peking University Health Science Center, Beijing 100083, China. Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Apr;42(4):226-30
OBJECTIVE: To determine the relative molecular mass of marine collagen peptides (MCPs) and investigate the effects of MCPs on serum lipids, anti-oxidative enzymes and malondialdehyde (MDA) in hyperlipidemic rats.
METHODS: Sephadex G-25, high performance liquid chromatography (HPLC) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) methods were used to determine the relative molecular mass of MCPs. Then 50 healthy male SD rats were divided into 5 groups, which were normal control (NC) group, hyperlipidemic model control (HC) group and 1.0, 3.0, 9.0 g/kgbw MCPs groups, MCPs were orally administered by gavage to rats in MCPs group for 45 consecutive days (2 ml/100 kgbw per day), and the control rats were given vehicle only, all animals (except NC rats) were fed with a high fat diet composed of 79% basic diet, 10% lard, 10% yolk powder and 1% cholesterol. The levels of serum lipids, the content of MDA and activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in serum were measured.
RESULTS: The levels of serum total cholesterol (TC) in 1.0, 3.0, 9.0 g/kgbw MCPs groups were 1.89 +/- 0.29, 2.07 +/- 0.39 and 1.99 +/- 0.29 mmol/L respectively, each of which was significantly lower than that in HC group (3.37 +/- 0.24 mmol/L); low-density lipoprotein cholesterol (LDL-C) levels in 1.0, 3.0, 9.0 g/kgbw MCPs groups were 0.83 +/- 0.16, 1.01 +/- 0.35 and 0.91 +/- 0.26 mmol/L respectively, each of which was significantly lower than that in HC group(2.20 +/- 0.34 mmol/L); triglyceride (TG) levels in 3.0 and 9.0 g/kgbw MCPs groups (0.90 +/- 0.15 and 0.86 +/- 0.12 mmol/L) were reduced significantly compared with that in HC group (1.18 +/- 0.18 mmol/L); MDA level in 9.0 g/kgbw MCPs group was 7.1 +/- 4.1 nmol/ml, which was significantly lower than that in HC group ( 15.9 +/- 9.9 nmol/ml); and atherogenic index (AI) in hyperlipidemic rats fed with 1.0, 3.0, 9.0 g/kgbw MCPs were 1.14 +/- 0.22, 1.16 +/- 0.27 and 0.99 +/- 0.31 respectively, each of which was significantly lower than that in HC group (2.27 +/- 0.55). The activities of SOD in 1.0, 3.0, 9.0 g/kgbw MCPs groups (218.6 +/- 33.2, 242.7 +/- 21.4 and 242.1 +/- 44.8 U/ml) were obviously increased compared with that in HC group (119.7 +/- 47.8 U/ml), and anti-atherogenic index (AAI) were also increased significantly (0.47 +/- 0.04, 0.47 +/- 0.06, 0.51 +/- 0.09 vs 0.31 +/- 0.05).
CONCLUSION: MCPs should have antioxidative and lipid-lowering effects, and might play a preventive role in hyperlipidemia and atherogenesis.
Berberine prevents UV-induced MMP-1 and reduction of type I procollagen expression in human dermal fibroblasts.
Kim S, Chung JH. Phytomedicine. 2008 Sep;15(9):749-53. Epub 2007 Dec 27.
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea. ksm3005@hotmail.com
Matrix metalloproteinases (MMPs) induction and type I procollagen reduction in photoaging of the skin due to exposure to ultraviolet (UV) irradiation. Therefore, regulation of these genes has been suggested to be a useful tool to abolish skin aging. In this study, antioxidative plant ingredients used in traditional Chinese medicine, berberine (BBR) was investigated for their capacity to regulate MMP-1 and type I procollagen expression in human dermal fibroblasts. Our results showed that both basal and UV-induced MMP-1 expression was decreased by BBR. On the other hand, type I procollagen expression was dose-dependently increased by it. In addition, UV-induced reduction of type I procollagen expression is recovered by it. Therefore, we suggest that BBR may be a possible candidate for anti-skin aging agent.